Yeah Sure. However, with such a small size and very little knowledge of your game, we're left to interpret what you've created. Everybody interprets things in a different way, I might see your character as a astronaut, somebody else might see it as a baby, but only you know its a mythical moon monster with a banjo. Get what I'm saying? What is the sprite, what's the basic story line or/and what will the character be doing. Create a mock up so we can better understand it.
I'm not sure how the tiles will look, you might find it looks a bit busy. Try to create a mock up, it'll help you and us to see how the tiles are working together and help us see what you want to achieve. Other than that there's not much anybody can say. Other than contrast in colours that is.
Oh, on the sprite, the backpack and the arm sometimes look like there one, which is a bit odd.
