Thank you all for the excellent suggestions, This being my first full tileset I figured I would have plenty of redo work to do. I was noticing that the contrast between the ground and the leaves was... rough..... I will definitely be refining all of that. Thank you for the suggestion of clumped grass eishiya that is a wonderful idea! Also I think the third rendering of the tree you did looks much better, the outlines I had on it made it look.. off..  I have been working in pyxel edit and I have not figured out how to mess with contrast in the program yet.. maybe I'm blind and have just overlooked the function... 

 MysteryMeat - I totally agree with you, I was thinking of making the "grass" tiles more like patches on a dirt background but have not yet mad a dirt tile. I was also thinking dirt would make the ferns look better too... or so i hope.