Very much different than your last orthographic mockup, which I really liked. But I really like this one too.
Some thoughts:
The roof looks good, a bit too contrasty though. Wood isn't that reflective. I would probably tone down the bright highlights a lot.
I think the little bush/small tree is too detailed. Too much noise going on. The normal sized trees suffer a bit from this too, they are quiet busy. I like the trunks.
Some of the cliff edges look strange, it either ends very abruptly when it touches grass, or blends into the dirt. I think you could make it better by adding border tiles for elevated ground.
I don't like the purple shadows on everything at all. The rocks may be an exception.
You have some perspective issues, I think. The trees and characters (both types) are both drawn at a side view, and the roof seems angled much more towards the camera than in a traditional perspective. I don't think the back face should be visible. The rocks, ground and cliff look good. It might not hurt too add an extra pixel row on top of the fence and signs, either.
Another contrast Issue.. I think you're using way too much black here. On the house, the cast shadows, and the trees. It draws attention unnecessarily and isn't realistic. The cast shadow and the shadow on the roof of the house would imply the sun is directly to the hard left of the house, too.
As for the sprites, the left 2 seem to fit the style much better, but they are all out of perspective.