Scaling this up manually (and nonuniformly) really took its toll on the the pixel accuracy. No more even squares. (not needed on this forum either, we have on-click zoom features)
After scaling it down to what seems to be its accurate scale it is still bigger than 28x28, which is the allowed twitch emote resolution.
Also test your emote on both the dark and bright twitch chat color themes to make sure it looks good on both.