WOW. That is amazing. The run cycle is one of the best I've seen, and all the screenshots are gorgeous. I think the red one (top center) is my favorite, but the forests are in close competition.
I kind of prefer the old portrait at the moment, but I think the new one could beat it with a few more tweaks. It doesn't look like the style of the game generally thrives on the anime look, and some people might be put off by it, so a more original style would probably be better (I personally like anime style though).
The new new one is better than the old new one (

), but the eyes/eyebrows definitely need to be less sad (at least you lost the unibrow look). Also maybe switch back to the old body style. The new one is maybe a little more anatomically correct (old one her rib cage looks rather narrow), but she looks more like an ordinary office lady rather than the ass-kicking teenager type. And the difference in shading around the shoulder makes her arm look farther back, which doesn't seem to fit the personality for whatever reason.
Another thing, is that the skirt looks much tighter in the portrait than in the sprites. And that also makes her look older.
In any case, it's looking great already, so any changes will only be to further perfect

I dearly hope to play this game someday. One of the best looking I've seen in a long time. Are you doing the programming yourself too, or do you have a team, or are those just mockups so far?