That sure is some beautiful work. It's taken me a while to go through all those pages too.
You're developed the leaves so much, colour-wise, placement and shape yet I feel the trunk is being neglected, I think I personally have issues with the roots themselves, there seems to be no evidence that the roots ever go into the ground.

If you think about it realistically, the roots would be (at least) half submerged into the ground, I'd love to see some above like you have (maybe with a bit more detail, to reflect the amount of detail in the leaves) but also bumps in the grass where the roots go under. I feel like the Tree really needs to be held in place, by some clever shading showing the roots are there, without being shown.

hope this helps in some way. Just something different to think about I guess.

Big Update today, well long post at least.

Anyways, back to my SECOND pixel, I feel like I should tell you more about it.

My Friend and I were at work and our imagination runs wild while we're bored. We came up of the idea for a game called "Darkhest Hour" Obviously at first, we were both VERY ambition, thinking we're going to make this awesome 3D game with amazing graphics, kind of Devil May Cry like, after realizing we're just too young and too in experienced for that kind of thing, with neither of us knowing how to do any kind of 3d modeling or design which special wow programs on computers (sorry about my language, there, not that it was rude, just "special wow programs" wasn't very good )

So, Thinking about it a bit more, we decided a short DEMO version in Pixels would do the trick nicely, So I set off to make a background template with HUD and all that kinda thing, which you have already seen, and sadly hasn't developed much since =/

I'll give you a short description of the game soon, not today, my friend has yet to write it for me

Anyway, I have since made the main character, named: Nick Savage

Heres my very large unfinished sprite sheet:



I've yet to do

Jump
Take Damage
Death
Crouching with crossbow (yes, that's a crossbow)
And everything to do with a sword

I had ago at animations here,
Walk:


Walk with Crossbow:


This is all new to me and I can see things that just don't flow very well.

Any comments are greatly appreciated.

That is beautiful. I agree with Souly the watermill would look fantastic animated.

I'm not sure what else to say, I'd really like to see this develop. good luck with it

OMG I didn't save as Jpeg did I?! noob error 

-EDIT-

no no, it's PNG

anyways, thanks ndchristie, here's a quick edit: