The image has a lot of high brightness colors and not a lot of contrast. Both of these together make the sprite hard to read. You might want to improve the image before trying to animate everything.

Here are some things you could do to try to improve the image:
First you might want to have on a more neutral background. Colors tend to influence the colors around them so working on a bright green background can make the colors of the sprite look off.
Second you might want to add more contrast so you can tell the colors apart. Make a backup of the image and try picking color that are significantly darker. If you don't like how it turned out, you can always load the backup and try again.

Some things you can try to check your color choices:
Reset the zoom to 100% and see if the image is readable.
Check how the image looks good in grey scale.



After you do this, you might want to clean the image up a bit. It looks like you have some stray pixels on the legs and other places.


I just edited the colors to try to improve them. This might give you an idea of colors you can try.