I think it's much more trunk-like now! The form is much clearer and it looks round, and you've even got a nice twist to it near the bottom. It would look even better with a bit more contrast between the three browns, and maybe some more hue-shifting.
It looks a bit noisy to me still, though. At this scale, the bark texture is essentially noise, so I think you might be better off not depicting it, and instead focusing on the larger shapes, like the twist of the trunk, burrs, etc. If you prefer the noisy look, then I think you should avoid having the single-pixel highlights right next to the darkest brown, as it reads more like sharply jutting features (thorns?) than just bark texture. That's a good technique for something like a cactus or a studded mace, but doesn't look quite right on this tree.
A visual illustration of what I mean by it looking like it juts out too much:

The bottom sketches represent the apparent size of the bump represented by the different levels of contrast.
The left and middle sketches represent a mild bump like what you'd expect to find in the texture of a tree trunk, the right/high-contrast bump doesn't feel as natural for a trunk texture.

Also, the roots don't seem to go into the ground, they appear to end abruptly. That is probably because of the shadow/outline on the tops. Try not having the very tips shaded, it should look more like they smoothly enter the ground.

The shading around the edges makes it look pillow-shaded. Have you thought about your light source?

They might have some readability issues, and they lack any sense of flavour, there's nothing that makes them really feel like a part of this game, they're generic enough to be in any game. There are most likely visuals other than red-orange-green bars you can use to communicate these gameplay ideas. In the legal system especially! They love their paperwork, stamps, frustrated stares, and foot-tapping bailiffs! Think about what these mechanics are meant to represent. For example, I assume in the witness-pressing minigames, it represents pressing the witness when they look weak/distracted. Why not make THAT the actual gameplay, having their expressions or body language change? Depending on how your portraits are rigged (if at all), this could be a very easy thing to implement. I think that would be much more interesting to play than some generic mini-games, even though at its core it's the same thing - tap the screen at a predictable correct moment.

The anger bar colours overwhelm the judge's head. I think having the bright colours is important on this kind of bar to communicate their meaning, so you'll probably need to play with frames and changing the amount of colour visible. It also looks a bit lazy to have the exact same head sprite without changing its expression. I think having some happier and angrier judge faces could lend the game a lot more character, as long as that's not too silly for the game.
Why not have his changing face be a HUD item that's always there? The face could change, and it could be in a frame that also serves as a round happiness bar that loses sections as the judge gets angrier, and the remaining sections could become redder. That could take up less space than the horizontal bar, while allowing more space for his expression and happiness status.

The white line while pressing witnesses may be difficult to see, especially for people who don't see very well. I think a larger slider that glows (or otherwise changes visually) slightly while it's in the green zone would be much better. That would be slightly more accessible because it would provide more visual cues to the player about when to tap. A slight sound would help even more.

The red outer circle is again difficult to see, especially for people without perfect vision. Bright red, especially on black, in general is a terrible idea for a GUI, because it hurts people's eyes. I recommend using a different colour. I'd also recommend a larger circle so that it's easier to see and tap. Even if the circle's hitbox is actually larger than the circle, players will seek to tap the middle of the circle and will feel frustrated aiming for it even if they never actually miss.

The whole mast could probably be 2-4px thick and look fine. Just removing a couple of pixels from the current mast wouldn't help make it look reasonably-sized xP Look at some ref photos, like the ones Mystery Meat sent, pay attention to how thick (or rather, thin!) the masts are. They taper towards the top, and even at their thickest, they're only about as thick as a forearm. Those are all aluminium I think and with wood it would be a little different, but depending on the wood, it would probably either only a little bit thicker, or even thinner.

To make the box pop, I think you need to raise its edges. Or, if it's meant to be a small dip, then make the back wall longer so it looks like it's descending. I don't know which it's meant to be so here are lame edits for both:


While making those edits, I noticed you're using a lot of colours that aren't noticeable at 1x. You're just making more work for yourself by drawing things that can't be seen xP