Hello,
I'm developing a game but I have doubts on the scales the pixel, I'm doing in low resolution for the animations become more simple, I am a developer and I'm drawing pixel recently.

My main question is if I create a boss for example who is 3 times bigger than the main character, do I draw at the same resolution and scale or already drawing at the higher resolution? I need some tips   

In this GIF below the tles are drawn in 16x16and the character in 32x32.